electrophoresis (protein, PCR DNA/RNA fragment, DGGE, TGGE, etc.) is difficult. Information about band positions alone does not provide. Uji kuantitatif DNA dengan spektrofotometri UV-Vis, DNA murni dapat mengidentifikasi dan memurnikan fragmen DNA adalah elektroforesis gel agorose. Muhittin Yılmaz, Cem Ozic and İlhami Gok. Chapter 4 Discriminatory Power of Agarose. Gel Electrophoresis in DNA Fragments Analysis Seow Ven Lee and .

Molekul DNA menunjukkan polarisasi yang kuat sehingga memungkinkan baik gerak elektroforesis berdasarkan muatan negatifnya maupun gerak dielektroforesis berdasarkan induksi polarisasi. Perancangan alat menggunakan kombinasi prinsip elektroforesis dan dielektroforesis dilengkapi perangkat lunak untuk rna konsentrasinya sangat diperlukan.

Utamanya mengingat uji kualitatif DNA berbasis visualisasi pada gel elektroforesis bersifat sangat subyektif dan kurang terukur. Penelitian bertujuan untuk mendesain piranti untuk mengukur konsentrasi DNA berdasarkan visualisasinya pada gel elektroforesis menggunakan perangkat lunak berbasis MatLab.

Hasil penelitian menggunakan piranti tersebut memperlihatkan visualisasi DNA yang lebih optimal. Hasil pengukuran jumlah DNA menggunakan spektrofotometer memiliki kecenderungan yang sama dengan hasil pengukuran menggunakan perangkat lunak berbasis MatLab meskipun terdapat perbedaan nilai kuantitatif.

eleitroforesis Molecules of deoxyribo nucleic acid DNA show a strong ellektroforesis allowing for both motions of the dielectrophoresis induced by polarization and electrophoresis based on its negative charge. Considering high subjective and less quantifiable result of the visualization based qualitative test of DNA on gel electrophoresis, designing the tool using a combination of the principles of electrophoresis and dielectrophoresis completed with a software for optimization of DNA visualization and to measure the concentration of small and largesized DNA fragment is very needed.

The aim of this study was to design device for optimization of DNA visualization and measuring the eldktroforesis in the gel electrophoresis using MatLab- based software. The research results showed that the amount of DNA analysed using a spectrophotometer tend to similar with the measurement results using the MatLab-based software although there was differences in quantitative values.

Bray, J LewisM. Roberts, and JD Watson.

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Molecular Biology of The Cell. Short Protocols in Molecular Biology. Chou, Elekyroforesis, Tegenfeldt J.

Stochastic Relaxation, Gibbs distributions and Bayesian Restoration. Goldfarb D and Yin W Markov chain Monte Carlo methods for high dimensional inversion in remote sensing.

Agarose Gel Electrophoresis for the Separation of DNA Fragments

B 66 3 Review of bio-particle manipulation using dielectrophoresis. Nanyang Tech Univ, Singapore.

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water. Dielectrophoretic Manipulation of DNA: Ros and Anselmetti D.

The Elektrovoresis of Spatial Conformation. Molecular Cloning A Laboratory Manual. Tanaka K and N Yoshiike.

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Abstract Molekul DNA menunjukkan polarisasi yang kuat sehingga memungkinkan baik gerak elektroforesis berdasarkan muatan negatifnya maupun gerak dielektroforesis berdasarkan induksi polarisasi. Keywords DNA concentration; visualization; electrophoresis.

References Alberts B, D. B 66 3Hoeb M.